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Título : 78 kDa receptor for Man6P-independent lysosomal enzyme targeting: Biosynthetic transport from endoplasmic reticulum to “high-density vesicles”
Creador: González Noriega A
Nivel de acceso: Open access
Palabras clave : Cloruro de Amonio - uso terapéutico
Gradiente de Densidad -instrumentación
Retículo Endoplásmico - enzimología
Retículo Endoplásmico - metabolismo
Centrifugación por Gradiente de Densidad -instrumentación
Hepatocitos - enzimología
Hepatocitos - metabolismo
Lisosomas - enzimología
Lisosomas - metabolismo
Manosafosfatos - metabolismo
Transporte de Proteína - fisiología
Receptor IGF Tipo 2 - biosíntesis
Receptor IGF Tipo 2 - química - Receptor IGF Tipo 2 - metabolismo
Endosomas - enzimología
Endosomas - metabolismo
Ammonium Chloride - therapeutic use
Cell Line
Centrifugation, Density Gradient - intrumentation
Endoplasmic Reticulum - enzymology
Endoplasmic Reticulum -metabolism
Hepatocytes -enzymology
Hepatocytes - metabolism
Intracellular Fluid -metabolism
Lysosomes - enzymology
Lysosomes - metabolism
Mannosephosphates - metabolism
Protein Transport - physiology
Receptor, IGF Type 2 -biosynthesis
Receptor, IGF Type 2 - chemistry
Receptor, IGF Type 2 -metabolism
Endosomes - enzymology
Endosomes - metabolism
Manosa-6-fosfato-independient
focalización
Manosa receptor 6-fosfato
78 kDa del receptor
lisosómica
fraccionamiento subcelular
tráfico intracelular
Mannose 6-phosphate-independient
targeting
Mannose 6-phosphate receptor
78 kDa receptor
Lysosomal targeting
Subcellular fractionation
Intracellular trafficking
Descripción : Recent work has shown that the cation-independent mannose 6-phosphate and the 78 kDa receptors for lysosomal enzyme targeting are located in different cell compartments. While the mannose 6-phosphate receptor is enriched in the Percoll fractions that contain Golgi apparatus, most of the 78 kDa receptor is localized in a heavy fraction at the bottom of the Percoll gradient. This report presents the biosynthetic transport of the 78 kDa receptor. Newly synthesized 78 kDa receptor was transported to Golgi from endoplasmic reticulum with a half life of 5 min. From the Golgi apparatus, the receptor takes two routes; about 15–25% is transported to the plasma membrane, and the rest migrates to late endosomes, subsequently to prelysosomes and finally to the dense vesicles. The 78 kDa receptor starts appearing at the dense vesicles 120 min after biosynthesis and reaches a maximum of 40–50% of the total receptor. Treatment of cells with NH4Cl causes depletion of the receptor from the dense vesicles and prelysosomes and corresponding augmentation in endosomes and plasma membrane. These results suggest that the 78 kDa receptor cycles between compartments and that the dense vesicles seem to represent the most distal compartment in the biosynthetic pathway of this receptor
Colaborador(es) u otros Autores: González-Noriega A
Ortega Cuellar Dd
Michalak C
Fecha de publicación : 2006
Tipo de publicación: Artículo
Formato: PDF
Identificador del Recurso : 10.1016/j.yexcr.2005.12.019
Fuente: Exp Cell Res 312(7):1065-1078
URI : http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/1248
Idioma: eng
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