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Título : AMP-activated protein kinase regulates oxidative metabolism in Caenorhabditis elegans through the NHR-49 and MDT-15 transcriptional regulators
Creador: Moreno Arriola, Elizabeth
Nivel de acceso: Open access
Palabras clave : Aminoimidazol Carboxamida - análogos & derivados
Aminoimidazol Carboxamida -farmacología
Animales
Caenorhabditis elegans - Efectos de drogas
Caenorhabditis elegans - genética
Caenorhabditis elegans - metabolismo
Proteínas de Caenorhabditis elegans - genética
Proteínas de Caenorhabditis -metabolismo
Respiración de la Célula - Efectos de la droga
Metabolismo Energético - Efectos de drogas
Metabolismo Energético - genética
Ácidos Grasos - metabolismo
Regulación de la Expresión Génica
Glucosa - metabolismo
Humanos
Ácido Láctico - metabolismo
Longevidad - genética
Metformina - farmacología
Mitocondrias Efectos de drogas
Mitocondrias -metabolismo
Oxígeno - metabolismo
Consumo de Oxígeno - efectos de drogas
Subunidades de Proteína - genética
Subunidades de Proteína - metabolismo
Proteínas Serina-Treonina Quinasas - genética
Proteínas Serina-Treonina Quinasas - metabolismo
Receptores Citoplasmáticos y Nucleares - genética
Receptores Citoplasmáticos y Nucleares - metabolismo
Ribonucleótidos -farmacología
Transducción de Señal
Factores de Transcripción - genética
Factores de Transcripción - metabolismo
Transcripción Genética
Aminoimidazole Carboxamide - analogs & derivatives
Aminoimidazole Carboxamide - pharmacology
Animals
Caenorhabditis elegans - drug effects
Caenorhabditis elegans - genetics
Caenorhabditis elegans - metabolism
Caenorhabditis elegans Proteins - genetics
Caenorhabditis elegans Proteins - metabolism
Cell Respiration - drug effects
Energy Metabolism - drug effects
Energy Metabolism - genetics
Fatty Acids - metabolism
Gene Expression Regulation Glucose - metabolism
Humans
Lactic Acid - metabolism
Longevity - genetics
Metformin - pharmacology
Mitochondria - drug effects
Mitochondria - metabolism
Oxygen - metabolism
Oxygen Consumption - drug effects
Protein Subunits - genetics
Protein Subunits - metabolism
Protein-Serine-Threonine Kinases - genetics
Protein-Serine-Threonine Kinases - metabolism
Receptors, Cytoplasmic and Nuclear - genetics
Receptors, Cytoplasmic and Nuclear - metabolism
Ribonucleotides - pharmacology
Signal Transduction Transcription Factors - genetics
Transcription Factors - metabolism
Transcription, Genetic
Caenorhabditis elegans, Proteínas de C elegans, Subunidades de Proteína, Proteínas Serina-Treonina Quinasas, Receptores Citoplasmáticos y Nucleares, Factores de Transcripción, genética
Caenorhabditis elegans, Caenorhabditis elegans Proteins, Protein Subunits, Protein-Serine-Threonine Kinases, Receptors, Cytoplasmic and Nuclear, Transcription Factors, genetics
Descripción : Cellular energy regulation relies on complex signaling pathways that respond to fuel availability and metabolic demands. Dysregulation of these networks is implicated in the development of human metabolic diseases such as obesity and metabolic syndrome. In Caenorhabditis elegans the AMP-activated protein kinase, AAK, has been associated with longevity and stress resistance; nevertheless its precise role in energy metabolism remains elusive. In the present study, we find an evolutionary conserved role of AAK in oxidative metabolism. Similar to mammals, AAK is activated by AICAR and metformin and leads to increased glycolytic and oxidative metabolic fluxes evidenced by an increase in lactate levels and mitochondrial oxygen consumption and a decrease in total fatty acids and lipid storage, whereas augmented glucose availability has the opposite effects. We found that these changes were largely dependent on the catalytic subunit AAK-2, since the aak-2 null strain lost the observed metabolic actions. Further results demonstrate that the effects due to AAK activation are associated to SBP-1 and NHR-49 transcriptional factors and MDT-15 transcriptional co-activator, suggesting a regulatory pathway that controls oxidative metabolism. Our findings establish C. Elegans as a tractable model system to dissect the relationship between distinct molecules that play a critical role in the regulation of energy metabolism in human metabolic diseases. © 2016 Moreno-Arriola et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Colaborador(es) u otros Autores: Mohammed ELHafidi
Daniel Ortega-Cuéllar
Karla Carvajal
Fecha de publicación : 2016
Tipo de publicación: Artículo
Formato: pdf
Identificador del Recurso : 10.1371/journal.pone.0148089
Fuente: Plos One 11(1):1 - 20
URI : http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/1282
Idioma: eng
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