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Título : | Analysis of Cyp2b1 gene expression in the rat liver and brain by multiplex PCR |
Creador: | Vanoye Carlo, America |
Nivel de acceso: | Open access |
Palabras clave : | Citocromo P-450 CYP2B6 - uso terapéutico Citocromo P-450 CYP2B6 - metabolismo Citocromo P-450 CYP2B6 - toxicidad Citocromo P-450 CYP2B6 - análisis Citocromo P-450 CYP2B6 - química Química Encefálica - genética Química Encefálica - efectos de drogas Encéfalo - enzimología Encéfalo - metabolismo Encéfalo - efectos de drogas Hígado - efectos de drogas Hígado - enzimología Hígado - metabolismo Etanol - líquido cefalorraquídeo Etanol - química Etanol - toxicidad Expresión Génica - efectos de drogas Ratas Cytochrome P-450 CYP2B6 - therapeutic use Cytochrome P-450 CYP2B6 - metabolism Cytochrome P-450 CYP2B6 - toxicity Cytochrome P-450 CYP2B6 - analysis Cytochrome P-450 CYP2B6 - chemistry Brain Chemistry - genetics Brain Chemistry - drug effects Brain - enzymology Brain - metabolism Brain - drug effects Liver - drug effects Liver - enzymology Liver - metabolism Ethanol - cerebrospinal fluid Ethanol - chemistry Ethanol - toxicity Gene Expression - drug effects rats CYP450 Cyp2b1 Cyp2b2 Cerebro Hígado Multiplex RT-PCR expresión génica CYP450 Cyp2b1 Cyp2b2 Brain Liver Multiplex RT-PCR Gene expression |
Descripción : | The CYP2B6 enzyme is involved in the metabolism of environmental toxins and endogenous compounds. The expression and activity of the cyp2b6 gene is important for the brain’s ability to detoxify harmful compounds. In rats, the Cyp2b1 gene is homologous to the human Cyp2b6 gene and has an isoform, Cyp2b2, with nearly the same sequence. Although the distinction between them is fundamental, most studies report global changes in CYP2B enzymes due to the complexity of the techniques to detect both genes separately. We instrumented a semi-quantitative technique combining multiplex end point RT-PCR with restriction enzyme assays, allowing us to establish a relatively simple, reproducible and affordable method to distinguish Cyp2b1 gene expression from Cyp2b2. Using this technique, we showed differential Cyp2b1 expression after phenobarbital and ethanol induction in the brain and liver and detected increased Cyp2b1 expression after ethanol treatment in the brain striatum that had not been detected before. © 2015, The Korean Society of Toxicogenomics and Toxicoproteomics and Springer Science+Business Media Dordrecht. |
Colaborador(es) u otros Autores: | Gutiérrez-Ospina Gabriel Marcial-Quino Jaime Gómez-Manzo Saúl Garcia-Torres Itzhel De La Mora-De La Mora Ignacio Enríquez-Flores Sergio Méndez Sara T Phillips-Farfán Bryan V Gutiérrez-Castrellón Pedro |
Fecha de publicación : | 2015 |
Tipo de publicación: | Artículo |
Formato: | |
Identificador del Recurso : | 10.1007/s13273-015-0043-1 |
Fuente: | Molecular and Cellular Toxicology 11(4):407 - 414 |
URI : | http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/1404 |
Idioma: | eng |
Aparece en las colecciones: | Artículos |
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