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Título : Analysis of Cyp2b1 gene expression in the rat liver and brain by multiplex PCR
Creador: Vanoye Carlo, America
Nivel de acceso: Open access
Palabras clave : Citocromo P-450 CYP2B6 - uso terapéutico
Citocromo P-450 CYP2B6 - metabolismo
Citocromo P-450 CYP2B6 - toxicidad
Citocromo P-450 CYP2B6 - análisis
Citocromo P-450 CYP2B6 - química
Química Encefálica - genética
Química Encefálica - efectos de drogas
Encéfalo - enzimología
Encéfalo - metabolismo
Encéfalo - efectos de drogas
Hígado - efectos de drogas
Hígado - enzimología
Hígado - metabolismo
Etanol - líquido cefalorraquídeo
Etanol - química
Etanol - toxicidad
Expresión Génica - efectos de drogas
Ratas
Cytochrome P-450 CYP2B6 - therapeutic use
Cytochrome P-450 CYP2B6 - metabolism
Cytochrome P-450 CYP2B6 - toxicity
Cytochrome P-450 CYP2B6 - analysis
Cytochrome P-450 CYP2B6 - chemistry
Brain Chemistry - genetics
Brain Chemistry - drug effects
Brain - enzymology
Brain - metabolism
Brain - drug effects
Liver - drug effects
Liver - enzymology
Liver - metabolism
Ethanol - cerebrospinal fluid
Ethanol - chemistry
Ethanol - toxicity
Gene Expression - drug effects
rats
CYP450
Cyp2b1
Cyp2b2
Cerebro
Hígado
Multiplex RT-PCR
expresión génica
CYP450
Cyp2b1
Cyp2b2
Brain
Liver
Multiplex RT-PCR
Gene expression
Descripción : The CYP2B6 enzyme is involved in the metabolism of environmental toxins and endogenous compounds. The expression and activity of the cyp2b6 gene is important for the brain’s ability to detoxify harmful compounds. In rats, the Cyp2b1 gene is homologous to the human Cyp2b6 gene and has an isoform, Cyp2b2, with nearly the same sequence. Although the distinction between them is fundamental, most studies report global changes in CYP2B enzymes due to the complexity of the techniques to detect both genes separately. We instrumented a semi-quantitative technique combining multiplex end point RT-PCR with restriction enzyme assays, allowing us to establish a relatively simple, reproducible and affordable method to distinguish Cyp2b1 gene expression from Cyp2b2. Using this technique, we showed differential Cyp2b1 expression after phenobarbital and ethanol induction in the brain and liver and detected increased Cyp2b1 expression after ethanol treatment in the brain striatum that had not been detected before. © 2015, The Korean Society of Toxicogenomics and Toxicoproteomics and Springer Science+Business Media Dordrecht.
Colaborador(es) u otros Autores: Gutiérrez-Ospina Gabriel
Marcial-Quino Jaime
Gómez-Manzo Saúl
Garcia-Torres Itzhel
De La Mora-De La Mora Ignacio
Enríquez-Flores Sergio
Méndez Sara T
Phillips-Farfán Bryan V
Gutiérrez-Castrellón Pedro
Fecha de publicación : 2015
Tipo de publicación: Artículo
Formato: pdf
Identificador del Recurso : 10.1007/s13273-015-0043-1
Fuente: Molecular and Cellular Toxicology 11(4):407 - 414
URI : http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/1404
Idioma: eng
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