Por favor, use este identificador para citar o enlazar este ítem: http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/1437
Título : Analysis of POU5F1, c-Kit, PLAP, AP2y and SALL4 in gonocytes of patients with cryptorchidism
Creador: Vigueras Villaseñor, Rosa María
Nivel de acceso: Open access
Palabras clave : Adolescente
Biomarcadores - metabolismo
niño
Preescolar
Criptorquidismo - meatbolismo
Matriz Extracelular
Proteínas - metabolismo
Humanos
Lactante
Recién nacido
Masculino
Factor 3 de Transcripción de Unión a Octámeros - metabolismo
Proteínas Proto-Oncogénicas c-kit - metabolismo
Espermatozoides - metabolismo
Testículo - metabolismo
Testículo - patología
Factor de Transcripción AP-2 - metabolismo
Factores de Transcripción - metabolismo
Adolescent
Biomarkers - metabolism
Child
Child, Preschool
Cryptorchidism - metabolism
Extracellular Matrix Proteins - metabolism
Humans
Infant
Infant, Newborn
Male
Octamer Transcription Factor-3 - metabolis
Proto-Oncogene Proteins c-kit - metabolism
Spermatozoa - metabolism
Testis - metabolism
Testis - pathology
Transcription Factor AP-2 - metabolism
Transcription Factors - metabolism
AP2γ
Criptorquidia
Gonocitos
Neoplasia de células germinales intratubulares no clasificada
PLAP
POU5F1
SALL4
C-kit
AP2γ
Cryptorchidism
Gonocytes
Intratubular germ cell neoplasia unclassified
PLAP
POU5F1
SALL4
c-Kit
Descripción : Cryptorchidism is a risk factor for the development of testicular germ cell tumors (TGCTs). The most common type of TGCT in cryptorchidism is seminoma. The intratubular germ cell neoplasia unclassified (ITGCNU) is a histological pattern preceding the development of seminomas and non-seminomas. It was suggested that in patients with cryptorchidism, the gonocytes remained undifferentiated with pluripotent abilities expressing proteins like POU domain class 5 transcription factor 1 (POU5F1), tyrosine kinase receptor c-Kit, placental-like alkaline phosphatase (PLAP), the transcription factor AP2γ and sal-like protein 4 (SALL4) that confer to the gonocytes this ability and therefore make them susceptible to develop ITGCNU. The aim of the present study was to determine if the gonocytes of patients with cryptorchidism express POU5F1, c-Kit, PLAP, AP2γ and SALL4 proteins after their differentiation period. Based on this, we evaluated samples of testicular tissue from newborns to 16-year old subjects with or without cryptorchidism in search of POU5F1, c-Kit, PLAP, AP2γ and SALL4 using immunocytochemical method, the results of which were validated by RT-PCR. The results showed that control subjects witnessed a down-regulation in the expression of these five proteins in the first year of life, which eventually disappeared. On the other hand, it was determined that 21.6% (8/37) of the patients with cryptorchidism continued to express, at least, one of the proteins analyzed in this study after the second year of life. And only 5.4% (2/37) of the patients were positive to the five markers. These data sustain the proposed hypothesis that in cryptorchid patients, ITGCNU arises from gonocytes that fail in their differentiation process to spermatogonia with conservation of the proteins (POU5F1, c-Kit, PLAP, AP2γ and SALL4) that maintain pluripotency and undifferentiated characteristics and which are responsible for making the gonocytes susceptible to malignancy. However, we cannot guarantee that these patients present neoplastic transformation. © 2015 Elsevier GmbH
Colaborador(es) u otros Autores: Cortés-Trujillo Lucero
Chávez-Saldaña Margarita
García Vázquez Francisco
Carrasco-Daza Daniel
Cuevas-Alpuche Osvaldo
Rojas-Castañeda Julio César
Fecha de publicación : 2015
Tipo de publicación: Artículo
Formato: pdf
Identificador del Recurso : 10.1016/j.acthis.2015.08.004
Fuente: Acta Histochemica 117(8):752 - 761
URI : http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/1437
Idioma: eng
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