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http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2159
Título : | Characterization of recombinant human cementum protein 1 (hrCEMP1): primary role in biomineralization |
Creador: | Villarreal Ramírez E |
Nivel de acceso: | Open access |
Palabras clave : | Calcificación Fisiológica Durapatita - química Fibroblastos - metabolismo Encía - citología Encía - metabolismo Glicosilación Humanos Fosforilación Estructura Secundaria de Proteína Proteínas - química Proteínas - genética Proteínas - metabolismo Proteínas Recombinantes - biosíntesis Proteínas Recombinantes - química Proteínas Recombinantes - genética Calcification, Physiologic Durapatite -chemistry Fibroblasts - metabolism Gingiva - cytology Gingiva - metabolism Glycosylation Humans Phosphorylation Protein Structure, Secondary Proteins - chemistry Proteins - genetics Proteins - metabolism Recombinant Proteins - biosynthesis Recombinant Proteins - chemistry Recombinant Proteins - genetics Cemento radicular, Biomineralización, Proteína 1 de cemento, Tejidos mineralizados, Hidroxiapatita, Fosfato octacalcium, Regeneración periodontal Cementum Biomineralization Cementum protein 1 Mineralized tissues Hydroxyapatite Octacalcium phosphate Periodontal regeneration |
Descripción : | Cementum protein 1 (CEMP1) has been recently cloned, and in vitro experiments have shown functions as regulator of cementoblast behavior and inducer of differentiation of non-osteogenic cells toward a cementoblastic/osteoblastic phenotype. In this study, we have produced a full-length human recombinant CEMP1 protein in a human gingival fibroblast cell line. The purified protein (hrCEMP1) has a M(r) 50,000. Characterization of hrCEMP1 indicates that its secondary structure is mainly composed of beta-sheet (55%), where random coil and alpha helix conformations correspond to 35% and 10%, respectively. It was found that hrCEMP1 is N-glycosylated, phosphorylated and possesses strong affinity for hydroxyapatite. Even more important, our results show that hrCEMP1 plays a role during the biomineralization process by promoting octacalcium phosphate (OCP) crystal nucleation. These features make CEMP1 a very good candidate for biotechnological applications in order to achieve cementum and/or bone regeneration |
Colaborador(es) u otros Autores: | Moreno A Mas-Oliva J Chávez-Pacheco JL Narayanan AS Gil-Chavarría I Zeichner-David M Arzate H. |
Fecha de publicación : | 2009 |
Tipo de publicación: | Artículo |
Formato: | |
Identificador del Recurso : | 10.1016/j.bbrc.2009.04.072 |
Fuente: | Biochem Biophys Res Commun 384(1):49-54 |
URI : | http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2159 |
Idioma: | eng |
Aparece en las colecciones: | Artículos |
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