Por favor, use este identificador para citar o enlazar este ítem: http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2197
Título : Clusters of biotin-responsive genes in human peripheral blood mononuclear cells
Creador: Silke Wiedmann
Nivel de acceso: Open access
Palabras clave : Biotina - Sangre
Biotina - Farmacología
Nucleo celular - Metabolismo
Celulas cultivadas
Concanavalina A - Farmacología
Citoplasma - Metabolismo
Expresión Génica - Efectos de drogas
Leucocitos Mononucleares - Efectos de drogas
Leucocitos Mononucleares - Metabolismo
Análisis de Secuencia por Matrices de Oligonucleótidos
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
Biotin - blood
Biotin - pharmacology
Cell Nucleus - Metabolism
Cells, Cultured
Concanavalin A - pharmacology
Cytoplasm - metabolism
Gene Expression - drug effects
Leukocytes, Mononuclear - drug effects
Leukocytes, Mononuclear - metabolism
Oligonucleotide Array Sequence Analysis
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction/genetics
Biotina
Microarrays de ADN
Expresión génica
Humanos
Células mononucleares de sangre periférica
Biotin
DNA microarrays
Gene expression
Human
Peripheral blood mononuclear cells
Descripción : Effects of biotin in cell signaling are mediated by transcription factors such as nuclear factor– B (NF- B) and Sp1/Sp3 as well as by posttranslational modifications of DNA-binding proteins. These signaling pathways play roles in the transcriptional regulation of numerous genes. Here we tested the hypothesis that biotin-dependent genes are not randomly distributed in the human genome but are arranged in clusters. Peripheral blood mononuclear cells were isolated from healthy adults before and after supplementation with 8.8 mol/day biotin for 21 days. Cells were cultured ex vivo with concanavalin A for 3 hours to stimulate gene expression. Abundances of mRNA encoding 14,000 genes were quantified by both DNA microarray and reverse transcriptase–polymerase chain reaction. The expression of 139 genes increased by at least 40% in response to biotin supplementation, whereas the expression of 131 genes decreased by at least 40% in response to biotin supplementation. The following clusters of biotin-responsive genes were identified: 1) 16% of biotin-responsive gene products localized to the cell nucleus; at least 28% of biotin-responsive genes play roles in signal transduction (these findings are consistent with a role for biotin in cell signaling); and 2) of the biotin-responsive genes, 54% clustered on chromosomes 1, 2, 3, 11, 12, and 19, whereas no biotin-responsive genes were found on chromosomes 10, 16, 18, 21, and heterosomes. This suggests that position effects play a role in biotin-dependent gene expression. Collectively, these findings suggest that the human genome contains clusters of biotin-dependent genes.
Colaborador(es) u otros Autores: Rodriguez Melendeza Rocio
Ortega Cuellara Daniel
Zempleni Janos
Fecha de publicación : 2004
Tipo de publicación: Artículo
Formato: pdf
Identificador del Recurso : 10.1016/j.jnutbio.2004.02.005
Fuente: J Nutr Biochem 15(7):433-439
URI : http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2197
Idioma: eng
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