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Título : Cyclosporin-A enhances non-functional axonal growing after complete spinal cord transection
Creador: Ibarra, Antonio
Nivel de acceso: Open access
Palabras clave : Axones - efectos de drogas - ratas
Ciclosporina - farmacología - ratas
Proteína GAP-43 - biosíntesis - ratas
Proteína GAP-43 - efectos de drogas - ratas
Inmunohistoquímica - métodos - ratas
Regeneración Nerviosa - efectos de drogas - ratas
Fármacos Neuroprotectores - farmacología - ratas
Tractos Piramidales - efectos de drogas - ratas
Ratas Sprague-Dawley
Traumatismos de la Médula Espinal - ratas
Axons - drug effects - rats
Cyclosporine - pharmacology - rats
GAP-43 Protein - biosynthesis - rats
GAP-43 Protein - drug effects - rats
Immunohistochemistry - methods - rats
Nerve Regeneration - drug effects - rats
Neuroprotective Agents - pharmacology - rats
Pyramidal Tracts - drug effects - rats
Rats, Sprague-Dawley
Spinal Cord Injuries - rats
GAP-43 Motor recovery Neuroregeneration Somatosensory evoked potential Spinal cord injury Therapy
Descripción : Therapeutic approaches that promote both neuroprotection and neuroregeneration would be valuable for spinal cord (SC) injury therapies. Cyclosporin-A (CsA) is an immunosuppressant that, due to its mechanism of action, could both protect and regenerate the neural tissue after injury. Previous studies have already demonstrated that intraperitoneal administration of CsA at a dose of 2.5 mg/kg/12 h during the first 2 days after SC contusion, followed by 5 mg/kg/12 h orally, diminishes tissue damage and improves motor recovery. In order to evaluate the effect of this CsA dosing regimen on axonal growth, we assessed motor recovery, presence of axons establishing functional connections and expression of GAP-43 in rats subjected to a complete SC transection. The Basso-Beattie-Bresnahan rating scale did not show difference in motor recovery of CsA or vehicle-treated rats. Moreover, somato-sensorial evoked potentials demonstrated no functional connections in the SC of these animals. Nevertheless, histological studies showed that: i) a significant number of CsA-treated rats presented growing axons, although they deviated perpendicularly at the edge of the stumps, surrounding them, ii) the expression of GAP-43 in animals treated with CsA was higher than that observed in the control group. Finally, anterograde tracing of the corticospinal tract of rats subjected to an incomplete SC transection showed no axonal fibers reaching the caudal stump. In summary, CsA administered at the dosing-regimen that promotes neuroprotection in SC contused rats induces both GAP-43 expression and axonal growth; however, it failed to generate functional connections in SC transected animals. © 2007 Elsevier B.V. All rights reserved.
Colaborador(es) u otros Autores: Edson Hernández
Joel Lomelic
Dante Pineda
Maribel Buenrostro
Susana Martiñón
Elisa Garcia
Nayeli Floresa
Gabriel Guizar Sahaguna
Dolores Correa
Ignacio Madrazo
Fecha de publicación : 2007
Tipo de publicación: Artículo
Formato: pdf
Identificador del Recurso : 10.1016/j.brainres.2007.02.056
Fuente: Brain Research 1149(1):200 - 209
URI : http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2233
Idioma: eng
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