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Título : Expression of Ik6 and Ik8 isoforms and their association with relapse and death in Mexican children with acute lymphoblastic leukemia
Creador: Reyes Leon, Adriana
Nivel de acceso: Open access
Palabras clave : Biomarcadores de Tumor - genética
Biomarcadores de Tumor - metabolismo
Niño
Preescolar
Femenino
Humanos
Factor de Transcripción Ikaros - genética
Factor de Transcripción Ikaros - metabolismo
Lactante
masculino
Leucemia-Linfoma Linfoblástico de Células Precursoras - diagnóstico
Leucemia-Linfoma Linfoblástico de Células Precursoras - metabolismo
Leucemia-Linfoma Linfoblástico de Células Precursoras - mortalidad
Pronóstico
Isoformas de Proteínas - genética
Isoformas de Proteínas - metabolismo
ARN Mensajero - genética
ARN Mensajero - metabolismo
Recurrencia
Biomarkers, Tumor - genetics
Biomarkers, Tumor - metabolism
Child
Child, Preschool
Female
Humans
Ikaros Transcription Factor - genetics
Ikaros Transcription Factor - metabolism
Infant
Male
Precursor Cell Lymphoblastic Leukemia-Lymphoma -diagnosis
Precursor Cell Lymphoblastic Leukemia-Lymphoma - metabolism
Precursor Cell Lymphoblastic Leukemia-Lymphoma - mortality
Prognosis
Protein Isoforms - genetics
Protein Isoforms - metabolism
RNA, Messenger - genetics
RNA, Messenger - metabolism
Recurrence
Marcadores de Tumor
Factor de Transcripción Ikaros
Leucemia Linfocítica Aguda
ARNm
Biomarkers, Tumor
Ikaros Transcription Factor
Acute Lymphocytic Leukemia
RNAm
Descripción : Expression of the 6 and 8 dominant-negative Ikaros isoforms in pediatric patients with acute lymphoblastic leukemia has been associated with a high risk of relapse and death; due to these isoforms disrupting the differentiation and proliferation of lymphoid cells. The aim of this study was to know the frequency of Ik6 and Ik8 in 113 Mexican ALL-children treated within the National Popular Medical Insurance Program to determine whether there was an association with relapse-free survival, event-free survival and overall survival, and to assess its usefulness in the initial stratification of patients. The expression of these isoforms was analyzed using specific primer sets and nested RT-PCR. The detected transcripts were classified according to the isoforms's sizes reported. A non-expected band of 300 bp from one patient was analyzed by sequencing. Twenty-six patients expressed Ik6 and/or Ik8 and one of them expressed a variant of Ik8 denominated Ik8-deleted. Although the presence of them was not statistically associated with lower relapse free survival (p = 0.432), event free survival (p = 0.667) or overall survival (p = 0.531), inferior overall survival was observed in patients that expressed these isoforms and showed high or standard risk by age and white blood-cell count at diagnosis. Of the 26 patients Ik6+ and/or Ik8+, 14 did not present adverse events; from them 6 were exclusively Ik6+ and/or Ik8+, and 8 were positive for the other Ikaros isoforms (Ik1, Ik2, Ik5, Ik3A, Ik4, Ik4A, Ik7). In the patients studied, the expression of Ik6 and Ik8 did not constitute an independent prognostic factor for relapse or death related to disease; therefore, they could not be used in the initial risk stratification. © 2015 Reyes-León et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Colaborador(es) u otros Autores: Juárez-Velázquez M
Medrano-Hernández A
Cuenca-Roldán T
Salas-Labadía C
Navarrete-Meneses MP
Rivera-Luna R
López-Hernández G
Paredes-Aguilera R
Pérez-Vera P
Fecha de publicación : 2015
Tipo de publicación: Artículo
Formato: pdf
Identificador del Recurso : 10.1371/journal.pone.0130756
Fuente: Plos One 10(7):1-12
URI : http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2419
Idioma: eng
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