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http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2478| Título : | Heterogeneity in the Activity of Mexican Helicobacter pylori Strains in Gastric Epithelial Cells and Its Association with Diversity in the cagA Gene |
| Creador: | Reyes Leon, Adriana |
| Nivel de acceso: | Open access |
| Palabras clave : | Antígenos Bacterianos - química - humanos - México Antígenos Bacterianos - genética - humanos - México Antígenos Bacterianos- metabolismo - humanos - México Proteínas Bacterianas - química - humanos - México Proteínas Bacterianas - genética - humanos - México Proteínas Bacterianas - metabolismo - humanos - México Células Epiteliales - Inmunología - humanos - México Células Epiteliales - microbiología - humanos - México Variación Genética - humanos - México Helicobacter pylori - microbiología - humanos - México Helicobacter pylori - clasificación - humanos - México Helicobacter pylori - genética - humanos - México Helicobacter pylori - aislamiento & purificación - humanos - México Helicobacter pylori - patogenicidad - humanos - México Interleucina-8 - biosíntesis - humanos - México Media edad - - humanos - México Datos de Secuencia Molecular - humanos - México Fenotipo - humanos - México Análisis de Secuencia de ADN - humanos - México Estómago - citología - humanos - México Estómago - Inmunología - humanos - México Antigens, Bacterial - chemistry - human - Mexico Antigens, Bacterial - genetics - human - Mexico Antigens, Bacterial - metabolism - human - Mexico Bacterial Proteins - chemistry - human - Mexico Bacterial Proteins - genetics - human - Mexico Bacterial Proteins - metabolism - human - Mexico Epithelial Cells - immunology - human - Mexico Epithelial Cells - microbiology - human - Mexico Genetic Variation - human - Mexico Helicobacter Infections - microbiology - human - Mexico Helicobacter pylori - classification - human - Mexico Helicobacter pylori - genetics - human - Mexico Helicobacter pylori - isolation & purification - human - Mexico Helicobacter pylori - pathogenicity - human - Mexico Interleukin-8 - biosynthesis - human - Mexico Middle Aged - human - Mexico Molecular Sequence Data - human - Mexico Phenotype - human - Mexico Sequence Analysis, DNA - human - Mexico Stomach - cytology - human - Mexico Stomach - immunology - human - Mexico |
| Descripción : | Helicobacter pylori CagA is translocated into gastric epithelial cells by a type IV secretion system and interacts with the Src homology 2 phosphatase, altering cell morphology. Multiple EPIYA motifs in CagA are associated with increased activity in cells and with gastric cancer. The aim of this work was to study the heterogeneity in activity in cells of multiple H. pylori single colonies isolated from a single patient and its association with polymorphism in cagA. The presence of cagA, cagE, cagT, and cag10 was studied with 318 H. pylori isolates from the antra and corpora of 18 patients. AGS gastric epithelial cells were infected with 75 isolates, and interleukin-8 (IL-8) secretion, cytoskeletal changes, CagA translocation, and tyrosine phosphorylation were measured. The cagA 3 -variable region was sequenced for 30 isolates to determine the number and types of EPIYA motifs. Isolates from an individual stomach were usually genetically related and had quantitatively similar phenotypic effects on cells (IL-8 induction and cytoskeletal changes). However, strains from different patients with similar CagA EPIYA motif patterns varied widely in these phenotypes. Among isolates with an EPIYA-ABC pattern, the phenotype was variable: IL-8 induction ranged from 200 to 1,200 pg/ml, and morphological changes occurred in 20 to 70% of cells. In several cases, cagA sequence diversity appeared to explain the lack of CagA activity, as isolates with an EPIYA-ACC pattern or a modified B motif had reduced cell activity. cag pathogenicity island-positive H. pylori isolates displayed a high level of heterogeneity in the capacity to induce IL-8 secretion and morphological changes; an absent or modified B motif was associated with low activity |
| Colaborador(es) u otros Autores: | John C. Atherton Richard H. Argent J. L. Puente J. Torres |
| Fecha de publicación : | 2007 |
| Tipo de publicación: | Artículo |
| Formato: | |
| Identificador del Recurso : | 10.1128/IAI.01951-06 |
| Fuente: | Infection and Immunity 75(7):3445-3454 |
| URI : | http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2478 |
| Idioma: | eng |
| Aparece en las colecciones: | Artículos |
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