Induction of CYP1A1 and CYP2E1 in rat liver by histamine: binding and kinetic studies

Davila Borja Victor Manuel,

Por favor, use este identificador para citar o enlazar este ítem: http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2529

Título :	Induction of CYP1A1 and CYP2E1 in rat liver by histamine: binding and kinetic studies
Creador:	Davila Borja Victor Manuel,
Nivel de acceso:	Open access
Palabras clave :	Citocromo P-450 CYP1A1 - efectos de drogas Citocromo P-450 CYP1A1 - metabolismo Histamina - farmacología Ratas Wistar Cytochrome P-450 CYP1A1 - drug effects Cytochrome P-450 CYP1A1 - metabolism Histamine - pharmacology Rats, Wistar histamina · CYP1A1 · CYP2E1 · Inducción · Inhibición catalítica Histamine · CYP1A1 · CYP2E1 · Induction · Catalytic inhibition
Descripción :	Histamine (HA) may bind to cytochrome P450 (CYP450) in rat liver microsomes. The CYP450-HA complex seems to regulate some cellular processes such as proliferation. In the present work, it is shown that HA increases the activity and protein level of CYP1A1 and CYP2E1, in vivo. CYP1A1 is associated with polycyclic aromatic hydrocarbon-mediated carcinogenesis and CYP2E1 with liver damage by oxidative stress. Studies of enzyme kinetics and binding with rat liver microsomes and supersomes® were carried out to determine whether HA is a substrate of CYP1A1 and/or CYP2E1. The lack of NADPH oxidation in the presence of HA showed that it is not a substrate for CYP1A1. Activity measurements using the O-dealkylation of ethoxyresoruWn indicated that HA is a mixed-type inhibitor of CYP1A1 in both microsomes and supersomes. On the other hand, HA induced a signiWcant NADPH oxidation catalyzed by CYP2E1 supersomes®, strongly suggesting that HA is a substrate for this isoform. Furthermore, HA is consumed in the presence of CYP2E1-induced microsomes and supersomes, as determined by o-phtalaldehyde complexes with HA by HPLC. The present Wndings may contribute to understand better the physiological function of CYP450 in relation with inXammation and other physiological processes in which HA may have a relevant role.
Colaborador(es) u otros Autores:	Dávila-Borja V Belmont J Espinosa J Moreno-Sánchez R Albores A Montero Rd
Fecha de publicación :	2007
Tipo de publicación:	Artículo
Formato:	pdf
Identificador del Recurso :	10.1007/s00204-007-0202-9
Fuente:	Archives of Toxicology 81(10):697-709
URI :	http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2529
Idioma:	eng
Aparece en las colecciones:	Artículos

Ficheros en este ítem:

No hay ficheros asociados a este ítem.