Por favor, use este identificador para citar o enlazar este ítem: http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2532
Título : Induction of immunity in sheep to Fasciola hepatica with mimotopes of cathepsin L selected from a phage display library
Creador: Villa Mancera A
Nivel de acceso: Open access
Palabras clave : Catepsinas - inmunología
Cisteína Endopeptidasas - inmunología
Fascioliasis - veterinaria
Biblioteca de Péptidos
Enfermedades de las Ovejas - inmunología
Cathepsins - immunology
Cysteine Endopeptidases - immunology
Fascioliasis - veterinary
Peptide Library
Sheep Diseases - immunology
Fasciola hepatica
catepsina L
mimotopes
vacuna
Fasciola hepatica
cathepsin L
mimotopes
vaccine
Descripción : An M13 phage random 12-mers peptide library was used to screen cathepsin L mimotopes of Fasciola hepatica and to evaluate their immunogenicity in sheep. Seven clones showed positive reactivity to a rabbit anti-cathepsin L1/L2 antiserum in ELISA, and their amino acid sequences deduced by DNA sequencing were tentatively mapped on the protein. Twenty sheep were randomly allocated into 4 groups of 5 animals each, for immunization with 1x10(14) phage particles of clones 1, 20, a mixture of 7 clones and PBS, without adjuvant at the beginning, and 4 weeks later. All groups were challenged with 300 metacercariae at week 6 and slaughtered 16 weeks later. The mean worm burdens after challenge were reduced by 47.61% and 33.91% in sheep vaccinated with clones 1 and 20, respectively; no effect was observed in animals inoculated with the clone mixture. Also, a significant reduction in worm size and burden was observed for those sheep immunized with clone 1. Animals receiving clone 20, showed a significant reduction in egg output. Immunization induced a reduction of egg viability ranging from 58.92 to 82.11%. Furthermore, vaccinated animals produced clone-specific antibodies which were boosted after challenge with metacercariae of F. hepatica.
Colaborador(es) u otros Autores: Quiroz-Romero H
 Correa D
 Ibarra F
 Reyes-Pérez M
 Reyes-Vivas H
 López-Velázquez G
 Gazarian K
 Gazarian T
 Alonso RA
Fecha de publicación : 2008
Tipo de publicación: Artículo
Formato: pdf
Identificador del Recurso : 10.1017/S003118200800471X
Fuente: Parasitology 135(12):1437 - 1445
URI : http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2532
Idioma: eng
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