Por favor, use este identificador para citar o enlazar este ítem:
http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2560| Título : | Kainate seizures increase nociceptin/orphanin fq release in the rat hippocampus and thalamus: a microdialysis study |
| Creador: | Carmona Aparicio, Liliana |
| Nivel de acceso: | Open access |
| Palabras clave : | Ácido Kaínico Ratas Sprague-Dawley Convulsiones - Químicamente inducidas Kainic Acid Rats, Sprague-Dawley Seizures - chemically induced Epilepsia kainato Microdiálisis Nociceptina Opioide Receptor como-1 Incautación epilepsy kainate microdialysis nociceptin opioid receptor like-1 seizure. |
| Descripción : | The neuropeptide nociceptin/orphanin FQ (N/OFQ) has been suggested to play a facilitatory role in kainate seizure expression. Furthermore, mRNA levels for the N/OFQ precursor are increased following kainate seizures, while its receptor (NOP) density is decreased. These data suggest increased N/OFQ release. To obtain direct evidence that this is the case, we have developed a microdialysis technique, coupled with a sensitive radioimmunoassay, that allows measurement of N/OFQ release from the hippocampus and thalamus of awake, freely moving animals. In both these brain areas, the spontaneous N/OFQ efflux decreased by approximately 50% and 65% when Ca2+ was omitted and when tetrodotoxin was added to the perfusion medium, respectively. Perfusion of the dialysis probe with high K+ increased N/OFQ release (approximately threefold) in a Ca2+-dependent and tetrodotoxin-sensitive manner. Kainate seizures caused a twofold increase in N/OFQ release followed, within 3 h, by a return to baseline levels. Approximately 5 h after kainate, a late increase in N/OFQ release was observed. On the following day, when animals were having only low grade seizures, N/OFQ release was not significantly different from normal. These phenomena were observed with similar patterns in the hippocampus and in the thalamus. The present data indicate that acute limbic seizures are associated with increased N/OFQ release, which may prime the molecular changes described above, i.e. cause down-regulation of NOP receptors and activation of N/OFQ biosynthesis |
| Colaborador(es) u otros Autores: | Candeletti Sanzio Binaschi Anna Mazzuferi Manuela Mantovani Simona Di Benedetto Manuela Landuzzi Daniela Lopetuso Giuseppe Omualdi Patrizia Simonato Michele |
| Fecha de publicación : | 2004 |
| Tipo de publicación: | Artículo |
| Formato: | |
| Identificador del Recurso : | 10.1111/j.1471-4159.2004.02633.x |
| Fuente: | J Neurochem 91(1):30-37 |
| URI : | http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2560 |
| Idioma: | eng |
| Aparece en las colecciones: | Artículos |
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.