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http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2575| Título : | Lectin from Phaseolus acutifolius var. escumite: Chemical characterization, sugar specificity, and effect on human T-lymphocytes |
| Creador: | Castillo Villanueva, Adriana |
| Nivel de acceso: | Open access |
| Palabras clave : | Secuencia de Aminoácidos - fisiología Carbohidratos - análisis Carbohidratos - química División Celular - efectos de drogas Galactosa - química humanos Datos de Secuencia Molecular Phaseolus - química Lectinas de Plantas - química Lectinas de Plantas - metabolismo Lectinas de Plantas - farmacología Homología de Secuencia Linfocitos T - efectos de drogas Amino Acid Sequence - physiology Carbohydrates - analysis Carbohydrates - chemistry Cell Division - drug effects Galactose - chemistry Humans Molecular Sequence Data Phaseolus - chemistry Plant Lectins - chemistry Plant Lectins - metabolism Plant Lectins - pharmacology Sequence Homology T-Lymphocytes - drug effects Phaseolus acutifolius var. escumite lectins glycoproteins lectin sugar specificity mitogenic lectin |
| Descripción : | Purification of the lectin from Phaseolus acutifolius var. escumite was achieved by affinity chromatography on a column containing glutaraldehyzed membranes from blood group O erythrocytes. The lectin is a tetrameric glycoprotein of 121 kDa with 10% of sugar by weight composed by four subunits of 30 kDa as determined by SDS-PAGE. The lectin is composed of four isolectins as determined by ion-exchange chromatography on a mono-S column. The lectin and its isolectins showed identical NH2 terminal residues (ANDLSFNFQR FNETN) with homology to the PHA leucoagglutinin-precursor. Peptide mass fingerprint from each lectin isoform determined from tryptic peptides by MALDI-TOF (matrix assisted laser desorption ionization-time-of-flight) showed differences among subunits, thus suggesting microheterogeneity in their amino acid sequences or different glycosylation patterns. The lectin and its four isolectins agglutinated erythrocytes without serological specificity and showed mitogenic activity on human leukocytes; moreover, the main effect was rather toward CD8+ than to CD4+ human peripheral lymphocytes. The lectin from escumite was not inhibitable by simple sugars; however, the specificity of the lectin and its isoforms was mainly addressed toward galactose residues present in bi- or triantennary N-acetyllactosamine-type glycans |
| Colaborador(es) u otros Autores: | Heriberto Caballero-Ortega Fikrat Abdullaev-Jafarova Yonathan Garfias Maria del Carmen Jiménez-Martínez Stephane Bouquelet Gisela Martínez Guillermo Mendoza-Hernández Edgar Zenteno |
| Fecha de publicación : | 2007 |
| Tipo de publicación: | Artículo |
| Formato: | |
| Identificador del Recurso : | 10.1021/jf063644k |
| Fuente: | Journal of Agricultural and Food Chemistry 55(14):5781 - 5787 |
| URI : | http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2575 |
| Idioma: | eng |
| Aparece en las colecciones: | Artículos |
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