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Título : Molecular identification of yeast of the pulque by pcr-dgge, a traditional mexican beverage
Creador: Marcial Quino Jaime
Nivel de acceso: Open access
Palabras clave : Agave - metabolismo
Agave - genética
Fermentación - génetica
Agave - química
Etanol - metabolismo
Etanol - química
Agave - metabolismo
Agave - genetics
Agave - chemistry
Fermentation - genetics
Ethanol - metabolism
Ethanol - chemistry
Pulque
PCR
Levadura
26SrRNA
Pulque
PCR
Yeast
26SrRNA
Descripción : Currently it is well known that yeasts play an essential role in the production of different beverages. In this paper, were identified some of the yeasts involved in the fermentation process of the pulque, a Mexican traditional beverage. Samples were collected from different regions of Mexico and yeasts were detected directly from samples without cultivation. Identifying the yeasts was obtained using amplification the D1/D2 domain of the 26S rRNA gene and Denaturing Gradient Gel Electrophoresis (DGGE). The results of DGGE showed different profiles of bands in each of the analyzed samples, indicating the presence of several species of yeast, which was also confirmed by sequencing of the bands corresponding to the domain D1/D2, succeeded in identifying five species of yeasts. The results obtained in thiswork demonstrated that the technique used for identification of yeasts of pulque was efficient. Besides, the optimization of this method could also allow rapid identification of yeasts and help understand the role of these in the fermentation process of this beverage, as well as theisolation of strains of interest for biotechnological purposes such as production of ethanol or metabolites with nutraceutical activity
Colaborador(es) u otros Autores: Garcia-Ocón B.
Mendoza-Espinoza J.A.
Gómez-Manzo S.
Enríquez-Flores S And Sierra-Palacios
Fecha de publicación : 2015
Tipo de publicación: Artículo
Fuente: International Journal of Research -GRANTHAALAYAH 3(3):1-15
URI : http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2611
Idioma: eng
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