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http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2624| Título : | MPTP decreases MT-I mRNA in mouse striatum |
| Creador: | Rojas, Patricia |
| Nivel de acceso: | Open access |
| Palabras clave : | Metil-4-fenil-1,2,3,6-Tetrahidropiridina - farmacología Cerebro - efectos de drogas Cerebro - metabolismo Corteza Cerebral -citología Corteza Cerebral -efectos de drogas Corteza Cerebral - metabolismo Cuerpo Estriado - citología Cuerpo Estriado - efectos de drogas Cuerpo Estriado - metabolismo Depuradores de Radicales Libres - metabolismo Expresión Génica - efectos de drogas Hibridación in situ - métodos Hígado - efectos de drogas 1 Hígado - metabolismo Envenenamiento por MPTP - inducido químicamente Envenenamiento por MPTP - fisiopatología Metalotioneína - análisis Metalotioneína -genética Metalotioneína - metabolismo Ratones Consanguíneos C57BL Neuronas - citología Neuronas - efectos de drogas Neuronas - metabolismo Estrés Oxidativo - efectos de drogas - ARN Mensajero - biosíntesis Sulfato de Zinc - farmacología Ratones Methyl-4-phenyl-1,2,3,6-tetrahydropyridine - pharmacology Brain - drug effects Brain - metabolism Cerebral Cortex - cytology Cerebral Cortex - drug effects Cerebral Cortex - metabolism Corpus Striatum - cytology Corpus Striatum - drug effects Corpus Striatum - metabolism Free Radical Scavengers - metabolism Gene Expression - drug effects In Situ Hybridization - methods Live - drug effects Liver - metabolism MPTP Poisoning - chemically induced 1 MPTP Poisoning -physiopathology Metallothionein - analysis Metallothionein - genetics Metallothionein -metabolism Mice, Inbred C57BL 2 Neurons - cytology Neurons - drug effects Neurons - metabolism oxidative Stress - drug effects RNA, Messenger - biosynthesis Zinc Sulfate -pharmacology Mice MPTP Núcleo lenticular farmacología ARNm Ratones MPTP Lenticular Nucleus phramacology mRNA Mice |
| Descripción : | 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) is a drug that induces parkinsonism in humans and non-human primates. Free radicals are thought to be involved in its mechanism of action. Recently, metallothionein has been proposed to play a role as a scavenger of free radicals. In the present work, we studied the effect of MPTP neurotoxicity on brain metallothionein-I (MT-I) mRNA expression. Male C-57 black mice were treated with MPTP (30 mg/kg, i.p., daily) for 3 or 5 days. All animals were killed by cervical dislocation 7 days after the last MPTP dose. The brains were removed quickly and immediately frozen, and quantitative in situ hybridization was performed using MT-I cDNA probe. MT-I mRNA content in striatum, a region which is known to be highly predisposed and sensitive to MPTP-induced oxidative stress, decreased by 30% (3 days) and 39% (5 days) respectively, after the last MPTP administration. These results suggest that MT-I gene expression is decreased in MPTP neurotoxicity. It is suggested that the reduction of MT, an anti-oxidant and a free radical scavenger, in the striatum by MPTP enables the neurotoxin to exert maximal oxidative damage to the striatum. |
| Colaborador(es) u otros Autores: | Rojas Castañeda J Vigueras RM Habeebu SS Rojas C Ríos C Ebadi M. |
| Fecha de publicación : | 2000 |
| Tipo de publicación: | Artículo |
| Formato: | |
| Identificador del Recurso : | 10.1023/A:1007564126478 |
| Fuente: | Neurochemical Research 25(4):503 - 509 |
| URI : | http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2624 |
| Idioma: | eng |
| Aparece en las colecciones: | Artículos |
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