Por favor, use este identificador para citar o enlazar este ítem: http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2648
Título : NFE2L2 Gene Variants and Arsenic Susceptibility: A Lymphoblastoid Model
Creador: Morales Marin ME
Nivel de acceso: Open access
Palabras clave : Arsénico - toxicidad
Proliferación Celular - Efectos de drogas
Proliferación Celular - genética
Contaminantes Ambientales - toxicidad
Regulación de la Expresión Génica - Efectos de drogas
Genotipo
Humanos
Linfocitos B - citología
NAD(P)H Deshidrogenasa (Quinona) - genética
NAD(P)H Deshidrogenasa (Quinona) - metabolismo
Factor 2 Relacionado con NF-E2 - genética
Factor 2 Relacionado con NF-E2 - metabolismo
Polimorfismo de Nucleótido Simple
Reacción en Cadena en Tiempo Real de la Polimerasa
Arsenic - toxicity
Cell Proliferation - drug effects
Cell Proliferation - genetics
Environmental Pollutants - toxicity
Gene Expression Regulation - drug effects
Genotype
Humans
Lymphocytes - cytology
NAD(P)H Dehydrogenase (Quinone) - genetics
NAD(P)H Dehydrogenase (Quinone) - metabolism
NF-E2-Related Factor 2 - genetics
NF-E2-Related Factor 2 - metabolism
Polymorphism, Single Nucleotide
Real-Time Polymerase Chain Reaction
Arsénico
Contaminantes Ambientales
Nuclear Factor E2 Related Factor 2
SNPs
Arsenic
Environmental Pollutants
NF-E2-Related Factor 2
Polymorphism, Single Nucleotide
Descripción : Arsenic (As) exposure is a major risk for several types of cancer and metabolic diseases such as diabetes. The transcription factor nuclear factor erythroid 2-related factor (Nrf2) is a key mediator in the cellular defense against As-induced adverse effects. The -653G/A and -617C/A gene variants modulate expression levels of the Nrf2 coding gene (NFE2L2) and are postulated to be associated with several illnesses. In this study the functional effect of these polymorphisms was investigated in the cellular sensitivity to As-mediated effects. Using quantitative real-time polymerase chain reaction (qRT-PCR) the basal levels of NFE2L2 mRNA and the induced levels of NFE2L2 and its target gene NQO1 were measured in lymphoblastoid cells carrying different genotypes for -653G/A and -617C/A polymorphisms following As exposure. The effects of different NFE2L2 gene genotypes on cell proliferation were also explored after chronic metal exposure. A tendency toward reduction in basal levels of NFE2L2 mRNA was noted in the heterozygous (GA/CA) and risk homozygous (AA/AA) genotypes of both polymorphisms in immortalized lymphoblastoid cells. Although the expression of NFE2L2 and NQO1 after acute acute iAs exposure was not markedly influenced by -653G/A and -617C/A genotype, it was found that these single-nucleotide polymorphisms (SNPs) were correlated with a differential sensitivity to chronic exposure to the metalloid. Further studies are needed to completely understand the role of -653G/A and -617C/A SNPs in regulation of the NFE2L2 gene.
Colaborador(es) u otros Autores: Mendez-García A
Centeno F
Martínez-Hernández A
Cordova EJ
Molina B
Frias S
Orozco L. 2015
Fecha de publicación : 2015
Tipo de publicación: Artículo
Formato: pdf
Identificador del Recurso : 10.1080/15287394.2015.1004146
Fuente: Journal of Toxicology and Environmental Health - Part A: Current Issues 78(10):628 - 634
URI : http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2648
Idioma: eng
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