Por favor, use este identificador para citar o enlazar este ítem:
http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2671| Título : | Partial bioenergetic characterization of Gluconacetobacter xylinum cells released from cellulose pellicles by a novel methodology |
| Creador: | Chavez Pacheco, Juan Luis |
| Nivel de acceso: | Open access |
| Palabras clave : | Celulosa Gluconacetobacter xylinus - Fisiología Microscopía Electrónica de Rastreo Cellulose Gluconacetobacter xylinus - Physiology Microscopy, Electron, Scanning - Methods |
| Descripción : | AIMS: Gluconacetobacter xylinum is well known for its ability to produce large amounts of cellulose, however, little is known about its cell physiology. Our goal was to study the respiratory metabolism and components of the respiratory system of this bacterium in static cultures. To reach our goal, a medium formulation had to be designed to improve cell growth and cellulose production together with a novel method for the recovery of cells from cellulose pellicles. METHODS AND RESULTS: Successive modifications of a nutrient medium improved G. xylinum cell growth 4.5-fold under static culture conditions. A blender homogenization procedure for the releasing of cells from the cellulose matrix gave a high yield of cells recovered. Respiratory activities of purified cells were greatly stimulated by exogenous substrates and showed to be resistant to KCN. Unexpectedly, exogenous NADH was oxidized at high rates. Cytochromes a, b, c and d were identified after spectral analyses. CONCLUSIONS: Partial bioenergetic characterization of G. xylinum cells allowed us to propose a scheme for its respiratory system. In addition, the growth medium for biomass production and the procedure for the efficient recovery of cells from cellulose pellicles were significantly improved. SIGNIFICANCE AND IMPACT OF THE STUDY: This work provides the first-ever bioenergetic characterization of G. xylinum grown in static cultures. In addition, a novel methodology to obtain purified cells in suitable quantities for biochemical research is described. |
| Colaborador(es) u otros Autores: | Martinez Yee S Contreras ML Gomez Manzo S Membrillo Hernandez J Escamilla JE |
| Fecha de publicación : | 2005 |
| Tipo de publicación: | Artículo |
| Formato: | |
| Identificador del Recurso : | doi:10.1111/j.1365-2672.2005.02708.x |
| Fuente: | J Appl Microbiol 99(5):1130-1140 |
| URI : | http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2671 |
| Idioma: | eng |
| Aparece en las colecciones: | Artículos |
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.