Por favor, use este identificador para citar o enlazar este ítem: http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2705
Título : Potential of HMEC-1 line and HUVEC primary culture cells to study the neonatal IgG Fc receptor in vitro
Creador: Ortiz Alegría Luz belinda
Nivel de acceso: Open access
Palabras clave : Células Endoteliales - immunology
Células Endoteliales - citología
Células Cultivadas - inmunología
Células Cultivadas - citología
ARN Mensajero - immunología
ARN Mensajero - ultraestructura
Proteínas
Microscopía
Inmunoglobulina G - immunología
Citometría de Flujo
Endothelial Cells - immunology
Endothelial Cells - cytology
Cells, Cultured - immunology
Cells, Cultured - cytology
RNA, Messenger - immunology
RNA, Messenger - ultrastructure
Proteins
Microscopy
Immunoglobulin G - immunology
Flow Cytometry
FcRn, células endoteliales, JUEGO-1, HUVECs
FcRn, Endothelial Cells, HMEC-1, HUVECs
Descripción : The neonatal IgG Fc receptor (FcRn) plays an important role in IgG homeostasis and immunity passive transfer. Fine points regarding these mechanisms, however, are still emerging. In order to obtain information about these phenomena, it is essential to have in vitro models of endothelium that express this receptor. In this study we chose two widely used models of human endothelial cells: the semi-immortalized and stable cell line HMEC-1 (CDC/USA) and the Human Umbilical Vein Endothelial Cells (HUVECs) which maintain morphological, phenotypical and functional characteristics of human micro and macro-vasculature endothelia, respectively. We found that both cells express the FcRn mRNA and protein using real-time RT-PCR, flow cytometry and confocal microscopy, respectively. We detected differences in mRNA expression levels in HUVECs among individuals. The protein was found on the cell surface but also intracellularly within vesicles. This study supports the use of two cell types as models of FcRn expression, allowing either to understand or to manipulate the mechanisms in which the receptor is involved in vivo. © 2016 Luz Belinda Ortiz-Alegria, Irma Canedo-Solares, Felipe Vadillo-Ortega, Marisol Castillo-Castrejon and Dolores Correa.
Colaborador(es) u otros Autores: Cañedo-Solares Irma
Vadillo-Ortega Felipe
Castillo-Castrejón Marisol
Correa Beltran María Dolores
Fecha de publicación : 2016
Tipo de publicación: Artículo
Formato: pdf
Identificador del Recurso : 10.3844/ajisp.2016.1.9
Fuente: American Journal of Immunology 12(1):1 -9
URI : http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2705
Idioma: eng
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