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Título : Serotonin Concentration, Synthesis, Cell Origin, and Targets in the Rat Caput Epididymis During Sexual Maturation and Variations Associated With Adult Mating Status: Morphological and Biochemical Studies
Creador: Jiménez Trejo, Francisco
Nivel de acceso: Open access
Palabras clave : Epidídimo- Citología - ratas
Epidídimo - enzimología - ratas
Epidídimo - metabolismo - ratas
Fenclonina - farmacología - ratas
Técnicas para Inmunoenzimas - ratas
Ratas Wistar
Receptores de Serotonina - metabolismo - ratas
Serotonina - Biosíntesis - ratas
Serotonina - metabolismo - ratas
Conducta Sexual Animal
Maduración sexual - ratas
Triptófano Hidroxilasa - antagonistas & inhibidores - ratas
Triptófano Hidroxilasa - metabolismo - ratas
Epididymis - cytology - rats
Epididymis - enzymology - rats
Epididymis - metabolism - rats
Fenclonine - pharmacology - rats
Immunoenzyme Techniques - rats
Rats, Wistar
Receptors, Serotonin - metabolism - rats
Serotonin - biosynthesis - rats
Serotonin - metabolism - rats
Sexual Behavior, Animal
Sexual Maturation - rats
Tryptophan Hydroxylase - antagonists & inhibitors - rats
Tryptophan Hydroxylase - metabolism - rats
5-hydroxytryptamine
tryptophan hydroxylase
neuroendocrine cells
male reproduction
serotonin receptor
spermatozoa.
Descripción : The caput epididymis of some mammals contains large quantities of serotonin whose origin, targets, and physiological variations have been poorly studied. We combined morphological and biochemical techniques to begin approaching these aspects of serotonin in the rat caput epididymis. Serotonin immunostaining was detected in mast, epithelial, and neuroendocrine cells. Epithelial cells displayed immunoreactivity to 5HT1A, 5HT2A, and 5HT3 serotonin receptors. Endothelial and mast cells labeled positive for 5HT1B serotonin receptors and spermatozoa displayed 5HT2A and 5HT3 serotonin receptor immunoreactivity. Epithelial, endothelial, and mast cells stained positive for serotonin transporters. Only epithelial cells showed tryptophan hydroxylase immunoreactivity; this enzyme catalyzes the limiting step in the serotonin synthetic pathway. In addition, Western blot analyses of caput homogenates documented the presence of 2 protein bands (<51 kd and <48 kd) that were immunoreactive for tryptophan hydroxylase. Chromatographic analyses documented the presence of tryptophan hydroxylase in the caput, and showed that both its activity and serotonin availability increased with sexual maturation and decreased following p-chlorophenylalanine treatment, an inhibitor of tryptophan hydroxylase activity. Interestingly, serotonin concentration and tryptophan hydroxylase activity tended to be higher in breeding males than in those with no mating experience. We think that these results support the existence of a local serotoninergic system in the rat caput epididymis that might regulate some aspects of male reproductive function
Colaborador(es) u otros Autores: Miguel Tapia-Rodríguez
Daniel B. C. Queiroz
Patricia Padilla
María Christina W. Avellar
Patricia Rivas Manzano
Gabriel Manjarrez-Gutiérrez
Gabriel Gutiérrez-Ospina
Fecha de publicación : 2007
Tipo de publicación: Artículo
Formato: pdf
Identificador del Recurso : 10.2164/jandrol.106.000653
Fuente: Journal of Andrology 28(1):136-149
URI : http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2801
Idioma: eng
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