Por favor, use este identificador para citar o enlazar este ítem: http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2917
Título : Toxicological evaluation of the natural products and some semisynthetic derivatives of Heterotheca inuloides Cass (Asteraceae)
Creador: Rodríguez Chávez, José Luis
Nivel de acceso: Open access
Palabras clave : Animales
Antineoplásicos - toxicidad
Artemia - Efectos de drogas
Asteraceae
Productos Biológicos -toxicidad
Línea Celular
Línea Celular Tumoral
Supervivencia Celular - Efectos de drogas
Femenino
Flores
Ratones
Pruebas de Toxicidad Aguda
Animals
Antineoplastic Agents - toxicity
Artemia - drug effects
Asteraceae
Biological Products - toxicity
Cell Line
Cell Line, Tumor
Cell Survival - drug effects
Female
Flowers
Mice
toxicity Tests, Acute
Heterotheca inuloides
Arnica mexicana
Citotoxicidad
Cadinanes
RAW264.7 macrófagos
Toxicidad aguda
Heterotheca inuloides
Mexican arnica
Cytotoxicity
Cadinanes
RAW264.7 macrophages
Acute toxicity
Descripción : Ethnopharmacological relevance Heterotheca ineuloides Cass (Asteraceae), popularly known as árnica mexicana, is widely used in Mexican traditional medicine to treat bruises, dermatological problems, rheumatic pains, and other disorders as cancer. The major constituents in H. inuloides are cadinane type sesquiterpenes, flavonoids and phytosterols. Compounds with a cadinane skeleton have been proved to possess cytotoxic activity against human-tumor cell lines and brine shrimp, and display toxic effects in different animal species. Although this plant has been widely used, there is little available information on the safety and toxicity especially of pure compounds. Aim of this study Evaluate the potential toxicity of the natural products isolated from H. inuloides and some semisynthetic derivatives. Materials and methods The toxic aspects of the following natural products isolated from dried flowers of H. inuloides: 7-hydroxy-3,4-dihydrocadalene (1), 7-hydroxycadalene (2), 3,7-dihydroxy-3(4H)-isocadalen-4-one (3), (1R,4R)-1-hydroxy-4H-1,2,3,4- tetrahydrocadalen-15-oic acid (4), D-chiro-inositol (5), quercetin (6), quercetin-3,7,3′-trimethyl ether (7), quercetin-3,7,3′,4′-tetramethyl ether (8), eriodictyol-7,4′-dimethyl ether (9), α-spinasterol (10), caryolan-1,9β-diol (11) and 7-(3,3-dimethylallyloxy)-coumarin (12) as well as the toxic aspects of the semisynthetic compounds 7-acetoxy-3,4-dihydrocadalene (13), 7-benzoxy-3,4-dihydrocadalene (14), 7-acetoxycadalene (15), 7-benzoxycadalene (16), quercetin pentaacetate (17), 7-hydroxycalamenene (18), 3,8-dimethyl-5-(1-methylethyl)-1,2-naphthoquinone (19), and 4-isopropyl-1,6-dimethylbenzo[c]oxepine-7,9-dione (20). Toxic activities of compounds were determined by sulforhodamine B (SRB) assay, Artemia salina assay, RAW264.7 macrophage cells. Additionally, the acute toxicity in mouse of compound 1, the major natural sesquiterpene isolated from the acetone extract, was evaluated. Results The best cytotoxicity activity was observed for mansonone C (19) on K562 cell line with IC50 1.45±0.14 μM, for 7-hydroxycadalene (2) on HCT-15 cell line with IC50 18.89±1.2 μM, and for quercetin pentaacetate (17) on MCF-7 cell line with IC50 22.57±2.4 μM. Sesquiterpenes mansonone C (19) and 7-hydroxy-3,4-dihydrocadalene (1) caused the strongest deleterious effects against A. salina with IC50 39.4±1.07, and 45.47±1.74 μM, respectively. The number of viable RAW 264.7 cells was reduced with sesquiterpenes 1 and 2 by more than 90%. In addition, the acute study of 1 revealed no lethal effects at 300 mg/kg body weight, however, a reduction in the body weight of mice, morphological changes in the tissues of the liver and kidney and toxic signs were observed at very high doses (2000 mg/kg). Conclusion The results provided evidence for the cytotoxicity of Mexican arnica (H. inuloides) metabolites and may be correlated with one of the popular uses of this plant, in traditional Mexican medicine, as anticancer remedy. Among the active compounds contained in the acetone extract, the cytotoxic activity is mainly ascribable to cadinene type sesquiterpenes. In addition, evidence of acute toxicity suggests that 7-hydroxy-3,4-dihydrocadalene (1) may lead to toxicity at very high doses. © 2015 Elsevier Ireland Ltd.
Colaborador(es) u otros Autores: Coballase-Urrutia Elvia
Sicilia-Argumedo Gloria
Ramírez-Apan Teresa
Delgado Guillermo
Fecha de publicación : 2015
Tipo de publicación: Artículo
Formato: pdf
Identificador del Recurso : 10.1016/j.jep.2015.08.055
Fuente: Journal of Ethnopharmacology 175():256 - 265
URI : http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2917
Idioma: eng
Aparece en las colecciones: Artículos

Ficheros en este ítem:
No hay ficheros asociados a este ítem.


Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.