Toxoplasmosis in pregnancy: determination of IgM, IgG and avidity in filter paper-embedded blood

Cañedo Solares, Irma

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Título :	Toxoplasmosis in pregnancy: determination of IgM, IgG and avidity in filter paper-embedded blood
Creador:	Cañedo Solares, Irma
Nivel de acceso:	Open access
Palabras clave :	Afinidad de Anticuerpos Ensayo de Inmunoadsorción Enzimática femeninos Humanos Inmunoglobulina G - sangre Inmunoglobulina M - sangre Complicaciones Parasitarias del Embarazo - diagnóstico Complicaciones Parasitarias del Embarazo - inmunologia Diagnóstico Prenatal Sensibilidad y Especificidad Pruebas Serológicas Toxoplasmosis - diagnóstico Toxoplasmosis - inmunologia Toxoplasmosis Congénita - prevención & control Antibody Affinity Enzyme-Linked Immunosorbent Assay Female Humans Immunoglobulin G/blood* Immunoglobulin M/blood* Pregnancy Pregnancy Complications, Parasitic/diagnosis* Pregnancy Complications, Parasitic/immunology Prenatal Diagnosis Sensitivity and Specificity Serologic Tests Toxoplasmosis/diagnosis* Toxoplasmosis/immunology Toxoplasmosis, Congenital/prevention & control avidez, IgM, IgG, recién nacidos, sangre de papel embebido en filtro, mujeres embarazadas avidity; IgM; IgG; newborns; paper filter-embedded blood; pregnant women
Descripción :	OBJECTIVE: To detect immunoglobulin M (IgM) anti-Toxoplasma gondii antibodies and determine immunoglobulin G (IgG) titer and avidity in filter paper-embedded blood (FPEB) samples of pregnant women. STUDY DESIGN: A total of 100 FPEB samples of pregnant women (30 positive and 70 negative) were analyzed for anti-T. gondii-specific IgM antibodies. Eleven and nine pairs of serum and FPEB samples were used to standardize IgG titration and avidity, respectively. Then, the correlation of avidity results was determined with 23 serum/FPEB pairs from IgG-positive cases. RESULT: IgM detection in FPEB was 92% sensitive and 100% specific. The titration of IgG antibodies in FPEB correlated with that of serum (r >or=0.9). Significant difference in avidity between the acute and the undetermined/chronic cases was observed in both samples. As expected, no correlation was found between IgM levels and avidity. CONCLUSION: The FPEB is useful to infer infection phase, and thus to speed clinical decisions in congenital toxoplasmosis management
Colaborador(es) u otros Autores:	Ortiz-Alegría LB Figueroa-Damián R Bustos-Bahena ML González-Henkel H Calderón-Segura E Luna-Pastén H Correa D.
Fecha de publicación :	2009
Tipo de publicación:	Artículo
Formato:	pdf
Identificador del Recurso :	10.1038/jp.2009.79
Fuente:	J Perinatol 29(10):668-72
URI :	http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2921
Idioma:	eng
Aparece en las colecciones:	Artículos

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